Rehydrating yeast

Yeast talk, turbo, bakers and specialised strains

Rehydrating yeast

Postby Furynfear » Fri Apr 11, 2014 6:34 am

Hey peeps,

I did a search on the forums and came up with 2 results. In spirits do u rehydrate yeast.

Also I making a tpw 2moro and was wondering if I need to rehydrate the yeast. Lowans Bakers yeast.
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby bt1 » Fri Apr 11, 2014 6:56 am

No gain for a Lowans, simply pitch directly to a well aerated fermenter at mid 20~ish temp and away you go. pays to circulate wash before adding to disperse yeast.

For specialist yeasts , EC1118 for example without rehydrating in plain water they lag or fail badly.

bt1
bt1
 
Posts: 2448
Joined: Sun Apr 22, 2012 11:56 am
Location: Adelaide
equipment: 2 x Glass Bubblers, 5 plate 89mm & 6 plate 110mm
4" 6 plate copper bubbler, 500mm copper packed section
Several pots, custom boiler
14 keg rotating brew setup, fermentation & dispenser fridges.

Re: Rehydrating yeast

Postby Furynfear » Fri Apr 11, 2014 7:16 am

What do you mean recirculate the wash.
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby bt1 » Fri Apr 11, 2014 8:35 am

simply stir it so it's moving... gradually add yeast, it will disperse better avoiding dry clumps of yeast, gives a more even start.

bt1
bt1
 
Posts: 2448
Joined: Sun Apr 22, 2012 11:56 am
Location: Adelaide
equipment: 2 x Glass Bubblers, 5 plate 89mm & 6 plate 110mm
4" 6 plate copper bubbler, 500mm copper packed section
Several pots, custom boiler
14 keg rotating brew setup, fermentation & dispenser fridges.

Re: Rehydrating yeast

Postby Yummyrum » Fri Apr 11, 2014 8:42 am

bt1 wrote:
For specialist yeasts , EC1118 for example without rehydrating in plain water they lag or fail badly.

bt1

Interesting point bt1 that could explain why I have had long lag times with EC1118

How long does it normally take to re-hydrate fully .My experience with hydrating was than most of the yeast just appeared to sink to the bottom and didn't really seem to change so after several hours I just pitched it and have never worried about it since........maybe I should
Yummyrum
 
Posts: 1897
Joined: Fri Dec 28, 2012 3:19 pm
Location: Near Coffs
equipment: Did use Still Spirits Reflux
Now use 50l Keg with Pot head for Rum and Neutral stripping runs on gas .
LM/VM head with 1m packed 2" on Still Spirits boiler for Neutrals.

Re: Rehydrating yeast

Postby OzKev » Fri Apr 11, 2014 9:13 am

Rehydrating yeast is just that, getting moisture into the cells. It should be limited to about 15mins, and you don't need a foaming monster at the end of it. Dropping to the bottom of the jar is ok. Rehydrating for too long can be detrimental to your yeast.

Creating a yeast starter is where you actively promote the yeast to take off fermenting.
OzKev
 
Posts: 356
Joined: Wed Oct 30, 2013 3:54 pm
Location: SE Qld
equipment: 50 L boiler with 2 x 2200w elements, and voltage controller
- Pot still with a 2" column, 1/2" liebig, with a few ogee attachments.
- 3" Modular Boka
Still Spirit Super Reflux
Still Spirit T500

All Grain brewery (BIAB, 1V and a 3v)
Temp controlled fermenting

Re: Rehydrating yeast

Postby Furynfear » Fri Apr 11, 2014 11:13 am

Well they say the minute you throw dry yeast in you kill half if u don't rehydrate. I no they replicate but was just curious if that's why he went 80grams in the tpw recipe. Maybe you could go 40g is you rehydrated the yeast.
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby Andy » Fri Apr 11, 2014 11:28 am

i always rehydrate my yeast, and I usually use Lowans. im not too sure bt1 thought behind it not needing it but ec-118 needing it

The suggested temperature from when a member emailed Lowans was 30 degrees, which is standard for most yeast.

Yummyrum- Rehydration only takes about 20mins- after several hours they would be starving!
Andy
 
Posts: 1466
Joined: Tue Aug 28, 2012 6:49 pm
Location: SOR, Perth
equipment: Copper Potstill on a 50L keg boiler with a 2200w element.
Doesn't get more simple then that

Re: Rehydrating yeast

Postby Furynfear » Fri Apr 11, 2014 1:22 pm

Andy wrote:i always rehydrate my yeast, and I usually use Lowans. im not too sure bt1 thought behind it not needing it but ec-118 needing it

The suggested temperature from when a member emailed Lowans was 30 degrees, which is standard for most yeast.

Yummyrum- Rehydration only takes about 20mins- after several hours they would be starving!


Hey mate how do u rehydrate the lowans. Step by step if u could. I've got the basics but a bit confused about it still
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby Furynfear » Fri Apr 11, 2014 1:32 pm

Watch "REHYDRATING DRIED YEAST" on YouTube
REHYDRATING DRIED YEAST: http://youtu.be/SL92Bd4kfbQ

What do u think of this.
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby Andy » Fri Apr 11, 2014 1:39 pm

i use about 2L of water. Ideally boil the water, then let it sit to cool down to 30 degrees. this is suppose to remove oxygen from it, but i usually just heat the water up to 30 degrees.
next poor the yeast in at let it sit in the warm water for 10-15mins. its best to do it in a pot that has a large surface area, otherwise they will just clump on top.
then give the yeast a swirl and let them sit for another 10-15 min.
then poor it into the wash.

I read a while ago that its best to rehydrate the yeast to plump up their cell walls before introducing them to the sugary environment, for this reason I don't add any sugar while rehydrating them (i know that some people do to start the yeast off)
Andy
 
Posts: 1466
Joined: Tue Aug 28, 2012 6:49 pm
Location: SOR, Perth
equipment: Copper Potstill on a 50L keg boiler with a 2200w element.
Doesn't get more simple then that

Re: Rehydrating yeast

Postby bt1 » Fri Apr 11, 2014 2:13 pm

Yum,

Had a fair bit of time with EC1118. Be wary the US market gold packeted product instructions sheet is not the same as the original spec sheet they send with batches to Aust.
I've settled on the following process based on the former spec sheet = matches my batch of EC1118.

Remove from fridge 15 mins prior to use
Spring water to 32c 200 300ml for 30gm = 60lt fermenter
Strongly aerate spring water, sprinkle yeast evenly across the swirling surface, it will sink as you mentioned.
After all sunk a very gentle stir, allow it 15mins. (More than 45min - hour yeast would stress with no food source).
Pitch temp must be within 5c to avoid thermal shock...
A gentle short stir into fermenter, lid off for first hour during early growth phase then lid on

Hope it helps
bt1
bt1
 
Posts: 2448
Joined: Sun Apr 22, 2012 11:56 am
Location: Adelaide
equipment: 2 x Glass Bubblers, 5 plate 89mm & 6 plate 110mm
4" 6 plate copper bubbler, 500mm copper packed section
Several pots, custom boiler
14 keg rotating brew setup, fermentation & dispenser fridges.

Re: Rehydrating yeast

Postby Yummyrum » Fri Apr 11, 2014 3:13 pm

Thanks for your replies.bt1 , why do you aerate water.Why does Andy think its best to have oxygen depleted water.
Yummyrum
 
Posts: 1897
Joined: Fri Dec 28, 2012 3:19 pm
Location: Near Coffs
equipment: Did use Still Spirits Reflux
Now use 50l Keg with Pot head for Rum and Neutral stripping runs on gas .
LM/VM head with 1m packed 2" on Still Spirits boiler for Neutrals.

Re: Rehydrating yeast

Postby OzKev » Fri Apr 11, 2014 6:05 pm

I think Andy meant that you are supposed to boil the water to sanitize it, but that depletes the oxygen which is not good, so he just heats his to 30c.

From here
https://www.wyeastlab.com/he-yeast-fundamentals.cfm

Oxygen is used by yeast for synthesis of sterols and unsaturated fatty acids that are necessary growth factors. Without oxygen, these lipids can’t be biosynthesized and growth will be very limited. The sterols and fatty acids produced are also very important in the structure of the cell membrane and the ability of the yeast to respond to external and internal stresses. Adequate oxygen in the wort when the yeast is pitched is essential to a successful fermentation and long-term health of the culture.
OzKev
 
Posts: 356
Joined: Wed Oct 30, 2013 3:54 pm
Location: SE Qld
equipment: 50 L boiler with 2 x 2200w elements, and voltage controller
- Pot still with a 2" column, 1/2" liebig, with a few ogee attachments.
- 3" Modular Boka
Still Spirit Super Reflux
Still Spirit T500

All Grain brewery (BIAB, 1V and a 3v)
Temp controlled fermenting

Re: Rehydrating yeast

Postby Andy » Fri Apr 11, 2014 6:15 pm

the theory behind rehydrating in oxygen depleted water is that the yeast will not be active during rehydration. you are aiming for plumping up their cells, not starting cell production. putting them in oxygenate water could be damaging as their cells membranes are still fragile- and oxygen after all is toxic. once these membrane has rehydrate, add them to an oxygen rich environment will help increase the strength of cell membrane, and start cellular activity- which is why we aerate our washes

keep in mind that all these factors don't matter TOO much. its mostly personal preference/habit.
Andy
 
Posts: 1466
Joined: Tue Aug 28, 2012 6:49 pm
Location: SOR, Perth
equipment: Copper Potstill on a 50L keg boiler with a 2200w element.
Doesn't get more simple then that

Re: Rehydrating yeast

Postby Yummyrum » Fri Apr 11, 2014 6:27 pm

OK so its one vote for oxygen and one vote against ......So I'll split the difference and just use some plain old filtered water at around close to the wash temp .Stick it in for 15-20 minuates then dump it in the wash :-D ........and pray :teasing-neener:
Yummyrum
 
Posts: 1897
Joined: Fri Dec 28, 2012 3:19 pm
Location: Near Coffs
equipment: Did use Still Spirits Reflux
Now use 50l Keg with Pot head for Rum and Neutral stripping runs on gas .
LM/VM head with 1m packed 2" on Still Spirits boiler for Neutrals.

Re: Rehydrating yeast

Postby Furynfear » Sat Apr 12, 2014 7:30 am

Yeast need oxygen to multiply. That's y u stir during rehydration
Furynfear
 
Posts: 85
Joined: Sat Apr 05, 2014 3:10 pm
equipment: T500. + python pot still + copper made reflux Still

Re: Rehydrating yeast

Postby bt1 » Sat Apr 12, 2014 8:01 am

Yum,

Just make sure your close to 30 - 35c I use 32c for rehydrating the temp is needed to activate the little beasties....can cool to wort temp gradually if needed by small additions periodically of cool water.

Fury I would not support stirring a yeast slurry to oxygenate it. You'll do more damage than good. The water should be well aerated prior
This assists with fatty acid coating they spray onto yeast grains breakdown-dissolving.

bt1
bt1
 
Posts: 2448
Joined: Sun Apr 22, 2012 11:56 am
Location: Adelaide
equipment: 2 x Glass Bubblers, 5 plate 89mm & 6 plate 110mm
4" 6 plate copper bubbler, 500mm copper packed section
Several pots, custom boiler
14 keg rotating brew setup, fermentation & dispenser fridges.

Re: Rehydrating yeast

Postby Andy » Sat Apr 12, 2014 12:40 pm

Furynfear wrote:Yeast need oxygen to multiply. That's y u stir during rehydration


correct- yeast need oxygen to multiply, but during rehydration they will not be multiplying. hence no oxygen required.
Andy
 
Posts: 1466
Joined: Tue Aug 28, 2012 6:49 pm
Location: SOR, Perth
equipment: Copper Potstill on a 50L keg boiler with a 2200w element.
Doesn't get more simple then that

Re: Rehydrating yeast

Postby OzKev » Sat Apr 12, 2014 12:48 pm

Yeast DO NOT need oxygen during their reproduction phase (log phase). Yeast use oxygen in their lag phase in the preparation for reproducing, which rehydration plays part of.
OzKev
 
Posts: 356
Joined: Wed Oct 30, 2013 3:54 pm
Location: SE Qld
equipment: 50 L boiler with 2 x 2200w elements, and voltage controller
- Pot still with a 2" column, 1/2" liebig, with a few ogee attachments.
- 3" Modular Boka
Still Spirit Super Reflux
Still Spirit T500

All Grain brewery (BIAB, 1V and a 3v)
Temp controlled fermenting

Next

Return to Yeast



Who is online

Users browsing this forum: No registered users and 14 guests

x