Yeast Propagation & Storage

Yeast talk, turbo, bakers and specialised strains

Yeast Propagation & Storage

Postby hjubm2 » Sun Apr 17, 2022 10:23 am

I've been thinking for awhile about starting to propagate & store yeast strains to be able to have whatever strain I want to use on hand & to be able to try some different ones that aren't commercially available. Is anyone propagating their own yeast & storing it long term?

I'm thinking of starting with a few commercial yeasts & isolating the single yeast cell & growing it out on a petri dish to then store it long term in the freezer. I've stored dried yeast this way but unsure if the yeast will survive in viable numbers if they are on a petri dish with agar. Or is there a better way to store the yeast? (Eg test tube with gelatin or similar?)

Then one they have been isolated I'll need to work out the best way to grow them out into a usable size without getting infection etc. This will be done by taking a small amount of the yeast from storage & growing it up in a low gravity, sterilised wort (say 20ml @ 1.01 gravity) & gradually building it up in size & strength until I have a useful starter.

Anyone got any advice or thoughts on this whole process? The main part will be making sure I can store the yeast long term & keep it viable without it dieing & keeping everything sterile while growing the yeast as when using a low number of cells it is too easy to get it infected or taken over by another strain.

Cheers guys
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Re: Yeast Propagation & Storage

Postby bluc » Sun Apr 17, 2022 10:48 am

See it done fair bit on the beer brewing forums. Lots good info on them. Check out home brew talk beer forum. Slant plates is one way they do it.
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Re: Yeast Propagation & Storage

Postby bluc » Sun Apr 17, 2022 10:52 am

This aint a bad read. Bit about glycerine may be of interest to you.
https://byo.com/article/yeast-ranching- ... 20freezing.
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Re: Yeast Propagation & Storage

Postby hjubm2 » Sun Apr 17, 2022 11:09 am

Thanks Bluc,

Have read a few of them articles from various beer places but everyone seems to say something different. I'll check that one out & see what extra info it gives me. Cheers mate
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Re: Yeast Propagation & Storage

Postby howard » Sun Apr 17, 2022 6:19 pm

i was thinking of going down the same path for a hard to get (and expensive) yeast i use for a certain beer.
watched a few you tubes.
the slant method seems quite easy. :shifty:
test tubes, glycerine etc.
interesting how most infect the glycerine over a heat source so there are no nasties are floating down.
then a bit of forward planning to step the yeast up from a small sample.
i saw a cheap way to make a stir plate with a cheap computer fan and a rare earth magnet.
plus a flask and stir bar obv.
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